Next Generation Sequencing Services: Our laboratory utilizes the Roche 454 FLX Genome Sequencer system (YES WE HAVE TITANIUM) . We offer comprehensive services for both contract and collaborative sequencing efforts. We have sequenced bacterial genomes, mammal and insect EST libraries, metagenome samples, cDNA libraries from bacteria, amplicon libraries, and viral genomes. We offer competative rates and low cost collaborative rates for researchers moving rapidly toward publications. Contact us for quotes on your next generation sequencing project.

info@researchandtesting.com

All pricing is based upon quotes!! We will provide general estimates but actual cost may vary. All quotes expire after 30 days except if otherwise agreed upon.

• For diversity analysis of low diversity samples (e.g. skin) pricing can be as low as $100 per sample and generate thousands of sequences.
• For high diversity samples such as feces (if you want deep comprehensive diversity coverage) pricing can be several thousands of dollars per sample. We will help you determine the best strategy for your project.

We will consult with you on your project.

Kindly requested: To keep our prices low we do ask that you do not abuse our consulting and that you are not merely fact finding.

We are one of the most prolific laboratories in publishing next-generation sequence data.

CLICK HERE TO SEE SOME OF OUR PUBLICATIONS

• GETTING STARTED
• it's easy to start using our sequencing services. We specialize in microbial diversity sequencing and data analysis but we can handle most project that involve next generation sequencing using Roche FLX and Titanium 454 next generation sequencing technology.
• First, we help you set up an account if necessary with your institution. Next you prepare a project profile, which includes basic information about the samples you are submitting. We greatly appreciate as much information about your samples as possible. We can also email or snail mail you labels and instructions for preparing your sample for shipment. Once your sample arrives, it is entered into our LIMS system for tracking. Before, during and after the sequencing is completed, our technical support team is available to work with you.

  Bacteria
  Whole genome shotgun sequencing for de novo assembly or mapping. High quality draft assembly in weeks with paired end reads for scaffolding of contigs. Roche 454 Sequencing technology has been extensively proven with microrganisms using multiple species.

  Viruses
  Whole genome sequencing on viral isolates or Ultra Deep Sequencing™ process on mixed viral populations.

  Fungi
  Large fungal genome sequencing, cost-effective with the ultra-high throughput capabilities of 454 Sequencing process.

  Large eukaryotic genomes
  Sequencing of large organism genomes, previously unapproachable due to prohibitive cost and throughput limitations.

  PCR amplicons
  Sequencing of short PCR amplicons in a massively parallel fashion to uncover rare mutations. With 454 Sequencing technology each molecule/ amplicon within the mixture is sequenced individually allowing for the identification of rare variants and the assignment of haplotype information over the full sequenced length.

  Amplified tag sequencing (SAGE™, microRNA, ChIP pulldown, etc.)
  Sequencing of large pools of tag sequences to very high coverage. Through massive parallelization, the level of over-sampling allows frequency identification to very high accuracy.

  Environmental sequencing (Metagenomic samples)
  Sequence and perform diversity analysis of complex metagenomic samples. The strains in a mixture, if known, will be precisely identified.

  Population biology
  Screen differences between DNA in diseased and normal genomes or within species using 454 Sequencing technology.

  cDNA
  cDNA sequencing for de novo assembly of the transcriptome or mapping to a scaffold genome for gene discovery.

  BAC clones or pools
  De novo sequencing and assembly of your individual BACs or pool BACs to map it to a particular region of the genome.